標題: Spectrofluorometric assay for monoamine-preferring phenol sulfotransferase (SULT1A3)
作者: Lu, Lu-Yi
Hsu, Yen-Chang
Yang, Yuh-Shyong
生物科技學系
Department of Biological Science and Technology
公開日期: 15-Sep-2010
摘要: A continuous and real-time fluorometric assay for monoamine-preferring phenol sulfotransferase (SULT1A3) was developed. The methodology was based on the coupling of SULT1A1 to regenerate 3'-phosphoadenosine-5'-phosPhosulfate (PAPS) using 4-methylumbelliferyl sulfate (MUS) as a sulfuryl group donor. The fluorophore product (4-methylumbelliferone. MU) was continuously produced and monitored when SULT1A3 catalyzed dopamine sulfation with PAPS. The optimal conditions of this turnover reaction and substrate inhibition of SULT1A3 were also determined. This coupled-enzyme assay allows the continuous measurement of initial reaction velocity and the sensitivity is comparable to that of end-point radioactive isotope assay. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.ab.2010.06.001
http://hdl.handle.net/11536/32186
ISSN: 0003-2697
DOI: 10.1016/j.ab.2010.06.001
期刊: ANALYTICAL BIOCHEMISTRY
Volume: 404
Issue: 2
起始頁: 241
結束頁: 243
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