標題: In situ observation of cell-detachment process initiated by femtosecond laser-induced stress wave
作者: Maezawa, Yasuyo
Hosokawa, Yoichiroh
Okano, Kazunori
Matsubara, Mie
Masuhara, Hiroshi
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 1-Oct-2010
摘要: When a stress wave generated by focusing a femtosecond laser is loaded on an animal cell adhered on a substrate, the cell is detached from the substrate. There are two possible mechanisms for the cell detachment: (a) The cell is detached from a scaffold coated on a glass plate, and (b) the cell is detached from the glass plate with the scaffold. In this work, we have studied the cell-detachment mechanism by visualizing the scaffold with a fluorescence probe of quantum dots. When the cell was detached from the substrate, fluorescence from the scaffold simultaneously disappeared from the glass plate, although the scaffold was not irradiated by the laser. This indicates that detachment due to the stress wave is attributed to mechanism (a). On the other hand, when the cell was detached from the substrate by a trypsin treatment, the fluorescence from the scaffold remained, suggesting mechanism (b). By comparing both results, it is considered that physiological damage of the cell membrane during the detachment process by femtosecond laser-induced stress wave is less than that due to the trypsin treatment.
URI: http://dx.doi.org/10.1007/s00339-010-5771-4
http://hdl.handle.net/11536/32162
ISSN: 0947-8396
DOI: 10.1007/s00339-010-5771-4
期刊: APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
Volume: 101
Issue: 1
起始頁: 127
結束頁: 131
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