標題: Neutron capture nuclei-containing carbon nanoparticles for destruction of cancer cells
作者: Hwang, Kuo Chu
Lai, Po Dong
Chiang, Chi-Shiun
Wang, Pei-Jen
Yuan, Chiun-Jye
生物科技學系
Department of Biological Science and Technology
關鍵字: Nanoparticles;Biocompatibility;Cell viability;Cell proliferation;Drug delivery
公開日期: 1-Nov-2010
摘要: HeLa cells were incubated with neutron capture nuclei (boron-10 and gadolinium)-containing carbon nanoparticles, followed by irradiation of slow thermal neutron beam. Under a neutron flux of 6 x 10(11) n/cm(2) (or 10 min irradiation at a neutron flux of 1 x 10(9) n/cm(2) s), the percentages of acute cell death at 8 h after irradiation are 52, 55, and 28% for HeLa cells fed with BCo@CNPs, GdCo@CNPs, and Co@CNPs, respectively. The proliferation capability of the survived HeLa cells was also found to be significantly suppressed. At 48 h after neutron irradiation, the cell viability further decreases to 35 +/- 5% as compared to the control set receiving the same amount of neutron irradiation dose but in the absence of carbon nanoparticles. This work demonstrates "proof-of-concept" examples of neutron capture therapy using (10)B-, (157)Gd-, and (59)Co-containing carbon nanoparticles for effective destruction of cancer cells. It will also be reported the preparation and surface functionalization of boron or gadolinium doped core-shell cobalt/carbon nanoparticles (BCo@CNPs, GdCo@CNPs and Co@CNPs) using a modified DC pulsed arc discharge method, and their characterization by various spectroscopic measurements, including TEM, XRD, SQUID, FT-IR, etc. Tumor cell targeting ability was introduced by surface modification of these carbon nanoparticles with folate moieties. (C) 2010 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.biomaterials.2010.07.057
http://hdl.handle.net/11536/32027
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2010.07.057
期刊: BIOMATERIALS
Volume: 31
Issue: 32
起始頁: 8419
結束頁: 8425
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