|標題:||Nanoparticle-enhanced Magnetic Field Induces Apoptosis in Nematode|
|作者:||Huang, G. S.|
Yeh, L. K.
Chen, Y. C.
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
|關鍵字:||apoptosis;nanoparticles;magnetic field;C. elegans|
|摘要:||To investigate molecular consequence of magnetic field to C. elegans, Fe3O4 nanoparticles were incorporated into the culture media of C. elegans and the nematodes were grown under magnetic field for 4 days. For the expermental group, we observed a 30 to 50 percents reduction in the moving speed. However, only nematode at L4 stage exhibited this difference. To explore molecular mechanisms affected by this treatment, real-time RT-PCR was performed using primers specific to amplify 120 genes. Differential expression for genes belong to apoptosis was observed. Biochemical assay for Caspase 3 activity was performed. The magnetic field-treated nematode exhibited high levels of Caspase 3 activity consistent with real-time PRC. Fluorescence microscopy showed that apoptotic activity was associated with the Fe3O4 treatment. For further validation, a ced-3 mutant nematode under enhanced magnetic field showed less effect for the movement than wild-type. The current study indicated that nanoparticle-enhanced magnetic field induced apoptosis in nematode.|
|期刊:||NSTI NANOTECH 2008, VOL 2, TECHNICAL PROCEEDINGS|