標題: Effective extraction and purification of beta-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioning
作者: Pan, IH
Yao, HJ
Li, YK
應用化學系
Department of Applied Chemistry
關鍵字: beta-xylosidase;aqueous two-phase system;purification;partitioning
公開日期: 1-二月-2001
摘要: Effective extraction of protein from bulk medium is an important technique in bioresearch. In the present study, we describe an extracellular beta -xylosidase from the fermentation supernatant of Trichoderma koningii G-39 that was successfully extracted and purified simultaneously in a single step by using an aqueous two-phase partitioning method. This two-phase system was prepared by dissolving suitable amount of poly(ethylene glycol) (PEG) and sodium dihydrogenphosphate (NaH2PO4) in aqueous solution. beta -Xylosidase was recovered with high yield and high concentration in the bottom salt-rich phase when 25% (w/v) PEG 1500 and 20-25% (w/v) NaH2PO4 were applied. Based on a 1-liter scale extraction, the purity of the enzyme was enhanced at least 33-fold. The total activity increased 422% in comparison with that in the untreated filtrate. The effectiveness and simplicity may make this technique potentially useful in various applications. The transxylosylation activity of the enzyme purified by this technique was also investigated. (C) 2001 Elsevier Science Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/S0141-0229(00)00291-X
http://hdl.handle.net/11536/29878
ISSN: 0141-0229
DOI: 10.1016/S0141-0229(00)00291-X
期刊: ENZYME AND MICROBIAL TECHNOLOGY
Volume: 28
Issue: 2-3
起始頁: 196
結束頁: 201
顯示於類別:期刊論文


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