標題: In-channel simplified decoupler with renewable electrochemical detection for microchip capillary electrophoresis
作者: Lin, Kuan-Wen
Huang, Yan-Kai
Su, Hsiu-Li
Hsieh, You-Zung
應用化學系
Department of Applied Chemistry
關鍵字: microchip capillary electrophoresis;electrochemical detection;decoupler;renewable electrode
公開日期: 30-Jun-2008
摘要: Electrochemical (EC) detection is comparable to fluorescence detection in that it is simple to perform, economical, and highly sensitive. In this study, we used replica molding to fabricate a PDMS microchip for microchip capillary electrophoresis (CE). A decoupler electrode and a working electrode were implanted into the PDMS chip during the molding process to prevent leakage into the electrode channel. The working electrode could be renewed readily through its slight withdrawal (ca. 3 mm) from the PDMS; its detection ability was highly reproducible in the microchip CE-EC system. The relative standard deviation (R.S.D.) of the detecting current for the renewed working electrode was 1.2% (n = 5). The calibration curves were linear for both dopamine and catechol analytes over the concentration range 10-1000 mu M, with coefficients ofdetermination (R-2) of 0.999 and 0.976, respectively. The number of theoretical plates (N/m) for these analytes was greater than 133,000. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.aca.2008.02.062
http://hdl.handle.net/11536/29353
ISSN: 0003-2670
DOI: 10.1016/j.aca.2008.02.062
期刊: ANALYTICA CHIMICA ACTA
Volume: 619
Issue: 1
起始頁: 115
結束頁: 121
Appears in Collections:Conferences Paper


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