標題: Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43
作者: Lai, YC
Lin, GT
Yang, SL
Chang, HY
Peng, HL
生物科技學系
Department of Biological Science and Technology
關鍵字: two-component system;response regulator;histidine kinase;Klebsiella gene
公開日期: 21-Jan-2003
摘要: A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/S0378-1097(02)01120-5
http://hdl.handle.net/11536/28157
ISSN: 0378-1097
DOI: 10.1016/S0378-1097(02)01120-5
期刊: FEMS MICROBIOLOGY LETTERS
Volume: 218
Issue: 1
起始頁: 121
結束頁: 126
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