標題: Blocking the dengue virus 2 infections on BHK-21 cells with purified recombinant dengue virus 2 E protein expressed in Escherichia coli
作者: Chiu, MW
Yang, YL
生物科技學系
Department of Biological Science and Technology
關鍵字: dengue virus;envelope (E) protein expression;functional assay;in vivo expression tag
公開日期: 26-Sep-2003
摘要: Dengue viruses (DVs) are mosquito-borne infectious pathogens. They have become an expanding public health problem in the tropics and subtropics. The dengue envelope (E) protein is one of the viral structure proteins responsible mainly for the virus attachment and entry onto host cells. It is also the major immunogen for virus neutralization. In this study, we have constructed a recombinant plasmid expressing a truncated E protein of DV-2 virus PL046 strain: The C-terminal hydrophobic domain of the E protein was removed and replaced with the sequence of S peptide to facilitate expression and purification. When expressed in Escherichia coli, the recombinant E proteins were found to be in the form of aggregated state. Through denaturation and dialysis processes, the receptor-interacting function of the purified recombinant E proteins was maintained, which was demonstrated by its ability to inhibit the DV-2 plaque-forming efficiency on mammalian BHK-21 host cells. (C) 2003 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bbrc.2003.08.053
http://hdl.handle.net/11536/27520
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2003.08.053
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 309
Issue: 3
起始頁: 672
結束頁: 678
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