標題: Gene delivery process in a single animal cell after femtosecond laser microinjection
作者: Hosokawa, Yoichiroh
Iguchi, Seriya
Yasukuni, Ryohei
Hiraki, Yuji
Shukunami, Chisa
Masuhara, Hiroshi
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Laser microinjection;Femtosecond laser;Gene expression;Cultured animal cell
公開日期: 30-Sep-2009
摘要: Microinjection of extracellular molecules into a single animal cell was performed by an amplified femtosecond laser irradiation. When a single-shot laser pulse was focused on the plasma membrane of a single fibroblast from the mouse cell line NIH3T3 with a high-numerical aperture objective lens, a transient hole with a diameter of 1 mm was formed. The delivery process of extracellular molecules immediately after the hole formation was monitored by a fluorescence staining with fluoresceini-sothiocyanate-dextran (FITC-dextran). Then the gene expression was confirmed using a DNA plasmid of an enhanced green fluorescent protein (EGFP). The gene expression was observed when the laser pulse was focused first on the cellular membrane and then on the nuclear membrane, while the gene was not expressed when the laser was focused only on the cellular membrane. On the basis of these results, the efficiency of gene delivery by the femtosecond laser microinjection and the subsequent gene expression were clarified. (C) 2009 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.apsusc.2009.04.111
http://hdl.handle.net/11536/27454
ISSN: 0169-4332
DOI: 10.1016/j.apsusc.2009.04.111
期刊: APPLIED SURFACE SCIENCE
Volume: 255
Issue: 24
起始頁: 9880
結束頁: 9884
Appears in Collections:Conferences Paper


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