標題: Desorption/ionization mass spectrometry on nanocrystalline titania sol-gel-deposited films
作者: Chen, CT
Chen, YC
應用化學系
Department of Applied Chemistry
公開日期: 2004
摘要: This paper describes a matrix-free method for performing desorption/ionization directly from mesoporous nanocrystalline titanic sol-gel thin films, which have good absorption capacity in the ultraviolet (UV) range and can act as assisting materials during UV matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analysis. A high concentration of citrate buffer was added into this system to provide the proton source and to reduce the presence of alkali cation adducts of the analytes. The analyte signals appear uniformly over the whole sample deposition area. Protonated molecules (MH+ ions) of analytes dominate the titania MALDI mass spectra. Surfactants, peptides, tryptic digest products, and small proteins with molecular weights below ca. 24 000 Da, are observed in the titania MALDI mass spectra. Detection limits for insulin are as low as ca. 2 fmol with mass resolution of ca. 660. Copyright (C) 2004 John Wiley Sons, Ltd.
URI: http://hdl.handle.net/11536/27232
http://dx.doi.org/10.1002/rcm.1572
ISSN: 0951-4198
DOI: 10.1002/rcm.1572
期刊: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume: 18
Issue: 17
起始頁: 1956
結束頁: 1964
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