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dc.contributor.authorWeng, MFen_US
dc.contributor.authorChen, YCen_US
dc.date.accessioned2014-12-08T15:39:51Z-
dc.date.available2014-12-08T15:39:51Z-
dc.date.issued2004en_US
dc.identifier.issn0951-4198en_US
dc.identifier.urihttp://hdl.handle.net/11536/27230-
dc.identifier.urihttp://dx.doi.org/10.1002/rcm.1501en_US
dc.description.abstractThis study demonstrates the feasibility of using sol-gel/crown ether hybrid materials as sample substrates that reduce the intensity of the signals of sodium ion adducts of oligonucleotides during matrix-assisted laser desorption/ionization (MALDI) analysis. 2-Hydroxymethyl[15]crown-5 and 2-hydroxymethyl[18]crown-6 were added as dopants during the sol-gel process to generate desalting substrates for MALDI sample deposition. The results demonstrate that the sol-gel/crown ether hybrid materials effectively suppress the formation of sodiated oligonucleotides during MALDI analysis. The largest detectable molecular size for an oligonucleotide was a 100-mer, and the detection limit for an oligonucleotide 36-mer was ca. 20 fmol. Copyright (C) 2004 John Wiley Sons, Ltd.en_US
dc.language.isoen_USen_US
dc.titleUsing sol-gel/crown ether hybrid materials as desalting substrates for matrix-assisted laser desorption/ionization analysis of oligonucleotidesen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/rcm.1501en_US
dc.identifier.journalRAPID COMMUNICATIONS IN MASS SPECTROMETRYen_US
dc.citation.volume18en_US
dc.citation.issue13en_US
dc.citation.spage1421en_US
dc.citation.epage1428en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000222376000002-
dc.citation.woscount14-
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