標題: Anti-HCV activities of selective polyunsaturated fatty acids
作者: Leu, GZ
Lin, TY
Hsu, JTA
生物科技學系
Department of Biological Science and Technology
關鍵字: HCV;replicon;polyunsaturated fatty acids including arachidonic acid;eicosapentaenoic acid;docosahexaenoic acid
公開日期: 21-May-2004
摘要: HCV infection can lead to chronic infectious hepatitis disease with serious sequelae. Interferon-alpha, or its PEGylated form, plus ribavirin is the only treatment option to combat HCV. Alternative and more effective therapy is needed due to the severe side effects and unsatisfactory curing rate of the current therapy. In this study, we found that several polyunsaturated fatty acids (PUFAs) including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) are able to exert anti-HCV activities using an HCV subgenomic RNA replicon system. The EC50 (50% effective concentration to inhibit HCV replication) of AA was 4 muM that falls in the range of physiologically relevant concentration. At 100 muM, alpha-linolenic acid, gamma-linolenic, and linoleic acid only reduced HCV RNA levels slightly and saturated fatty acids including oleic acid, myristic acid, palmitic acid, and steric acid had no inhibitory activities toward HCV replication. When AA was combined with IFN-alpha, strong synergistic anti-HCV effect was observed as revealed by an isobologram analysis. It will be important to determine whether PUFAs can provide synergistic antiviral effects when given as food supplements during IFN-based anti-HCV therapy. Further elucidation of the exact anti-HCV mechanism caused by AA, DHA, and EPA may lead to the development of agents with potent activity against HCV or related viruses. (C) 2004 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bbrc.2004.04.019
http://hdl.handle.net/11536/26762
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2004.04.019
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 318
Issue: 1
起始頁: 275
結束頁: 280
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