標題: Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43
作者: Chen, YT
Chang, HY
Lai, YC
Pan, CC
Tsai, SF
Peng, HL
生物科技學系
Department of Biological Science and Technology
關鍵字: rmpA;siderophore;heavy-metal resistance;tellurite resistance;iteron
公開日期: 4-Aug-2004
摘要: We have determined the entire DNA sequence of pLVPK, which is a 219-kb virulence plasmid harbored in a bacteremic isolate of Klebsiella pneumoniae. A total of 251 open reading frames (ORFs) were annotated, of which 37% have homologous genes of known function, 31% match the hypothetical genes in the GenBank database, and the remaining 32% are novel sequences. The obvious virulence-associated genes carried by the plasmid are the capsular polysaccharide synthesis regulator rmpA and its homolog rmpA2, and multiple iron-acquisition systems, including iucABCDiutA and iroBCDN siderophore gene clusters, Mesorhizobium loti fepBC ABC-type transporter, and Escherichia coli fecIRA, which encodes a Fur-dependent regulatory system for iron uptake. In addition, several gene clusters homologous with copper, silver, lead, and tellurite resistance genes of other bacteria were also identified. Identification of a replication origin consisting of a repA gene lying in between two sets of iterons suggests that the replication of pLVPK is iteron-controlled and the iterons are the binding sites for the repA to initiate replication and maintain copy number of the plasmid. Genes homologous with E. coli sopA/sopB and parA/parB with nearby direct DNA repeats were also identified indicating the presence of an F plasmid-like partitioning system. Finally, the presence of 13 insertion sequences located mostly at the boundaries of the aforementioned gene clusters suggests that pLVPK was derived from a sequential assembly of various horizontally acquired DNA fragments. (C) 2004 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.gene.2004.05.008
http://hdl.handle.net/11536/26484
ISSN: 0378-1119
DOI: 10.1016/j.gene.2004.05.008
期刊: GENE
Volume: 337
Issue: 
起始頁: 189
結束頁: 198
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