標題: Utilization of I kappa B-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-kappa B Inhibitor Activity
作者: Lin, Yu-Ling
Chen, Yen-Shun
Hsieh, Jui-Hung
Lin, Ching-Min
Wu, Hsin-Yi
Lin, Chen-Si
Chu, Rea-Min
Liao, Kuang-Wen
Hsu, Yuan-Hsun
生物科技學系
分子醫學與生物工程研究所
電子工程學系及電子研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
Department of Electronics Engineering and Institute of Electronics
關鍵字: Microbial metabolite;Antioxidant;I kappa B;EGFP;Hydroquinone;Propolis
公開日期: 1-十二月-2010
摘要: NF-kappa B regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-kappa B inhibitors have been discovered as an anti-tumor or anti-inflammatory drug. The activity of NF-kappa B transcription factor is negatively regulated by I kappa B binding. In this study, I kappa B assay system was established and I kappa B-EGFP fusion protein was used as an indicator to monitor the effects of substances on the I kappa B degradation. The results indicated that the chosen hydroquinone could inhibit the I kappa B degradation and cause the cell de-attachment from the bottom of culture plate. In addition, this system could also monitor the I kappa B degradation of microbial metabolite of natural mixtures of propolis. Thus, the I kappa B assay system may be a good system for drug discovery related to microbial metabolite.
URI: http://dx.doi.org/10.1007/s12575-010-9033-9
http://hdl.handle.net/11536/26285
ISSN: 1480-9222
DOI: 10.1007/s12575-010-9033-9
期刊: BIOLOGICAL PROCEDURES ONLINE
Volume: 12
Issue: 1
起始頁: 131
結束頁: 138
顯示於類別:期刊論文


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