標題: Interplay between Structure and Fluidity of Model Lipid Membranes under Oxidative Attack
作者: Tai, Wan-Yu
Yang, Yi-Cyun
Lin, Hui-Jen
Huang, Chin-Ping
Cheng, Yi-Lin
Chen, Mei-Fang
Yen, Hsiu-Lan
Liau, Ian
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 2-Dec-2010
摘要: A proper regula:ion of membrane fluidity is critical for cellular activities such as communication between cells, mitosis, an :I endocytosis. Unsaturated lipids, a main component of biological membranes, are particularly susceptible to oxidative attack of reactive oxygen species. The oxidation of lipids can produce structural derangement of membranes and eventually alter the membrane fluidity. We have applied fluorescence correlation spectroscopy (FCS) and Raman spectroscopy to investigate the fluidity and structure of model membranes subject to oxidative attack. Hydrogen peroxide has little effect on the lateral fluidity of membranes. whereas hydroxyl radical causes a significantly increased fluidity. The latter is rationalized with the cleavage of the acyl chains of lipids caused by hydroxyl radical; this interpretation is founded on the diminished intensities of lines in Raman spectra associated with -CH(2) and C = C moieties in lipids and supported by mass-spectral measurements. The same approach provides a mechanistic account of the inhibitory capability of vitamins C and E against ate increased membrane fluidity resulting from an oxidative attack. Membranes with much cholesterol exhibit a novel resistance against altered membrane fluidity induced with oxidative attack; this finding has biological implications. Our approach combining FCS and Raman measurements reveals the interplay between the structure and fluidity of membranes and provides insight into the pathophysiology of cellular oxidative injury.
URI: http://dx.doi.org/10.1021/jp1014719
http://hdl.handle.net/11536/26249
ISSN: 1520-6106
DOI: 10.1021/jp1014719
期刊: JOURNAL OF PHYSICAL CHEMISTRY B
Volume: 114
Issue: 47
起始頁: 15642
結束頁: 15649
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