標題: Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17 alpha/beta-exocyclic side chain stereochemistry
作者: Wu, Tung-Kung
Chang, Yi-Chun
Liu, Yuan-Ting
Chang, Cheng-Hsiang
Wen, Hao-Yu
Li, Wen-Hsuan
Shie, Wen-Shiang
生物科技學系
Department of Biological Science and Technology
公開日期: 2011
摘要: Site-saturated substitution in Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase at Ile705 position produced three chair-boat-chair (C-B-C) truncated tricyclic compounds, two 17 alpha-exocyclic protosteryl intermediates, two protosteryl C-17 truncated rearranged intermediates and the normal biosynthetic product, lanosterol. These results indicated the importance of the Ile705 residue in affecting lanosterol's C/D ring stabilization including 6-6-5 tricyclic and protosteryl C-17 cations and 17 alpha/beta-exocyclic side chain stereochemistry.
URI: http://hdl.handle.net/11536/26104
http://dx.doi.org/10.1039/c0ob00582g
ISSN: 1477-0520
DOI: 10.1039/c0ob00582g
期刊: ORGANIC & BIOMOLECULAR CHEMISTRY
Volume: 9
Issue: 4
起始頁: 1092
結束頁: 1097
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