標題: miRTarBase: a database curates experimentally validated microRNA-target interactions
作者: Hsu, Sheng-Da
Lin, Feng-Mao
Wu, Wei-Yun
Liang, Chao
Huang, Wei-Chih
Chan, Wen-Ling
Tsai, Wen-Ting
Chen, Goun-Zhou
Lee, Chia-Jung
Chiu, Chih-Min
Chien, Chia-Hung
Wu, Ming-Chia
Huang, Chi-Ying
Tsou, Ann-Ping
Huang, Hsien-Da
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
公開日期: 1-Jan-2011
摘要: MicroRNAs (miRNAs), i.e. small non-coding RNA molecules (similar to 22 nt), can bind to one or more target sites on a gene transcript to negatively regulate protein expression, subsequently controlling many cellular mechanisms. A current and curated collection of miRNA-target interactions (MTIs) with experimental support is essential to thoroughly elucidating miRNA functions under different conditions and in different species. As a database, miRTarBase has accumulated more than 3500 MTIs by manually surveying pertinent literature after data mining of the text systematically to filter research articles related to functional studies of miRNAs. Generally, the collected MTIs are validated experimentally by reporter assays, western blot, or microarray experiments with overexpression or knockdown of miRNAs. miRTarBase curates 3576 experimentally verified MTIs between 657 miRNAs and 2297 target genes among 17 species. miRTarBase contains the largest amount of validated MTIs by comparing with other similar, previously developed databases. The MTIs collected in the miRTarBase can also provide a large amount of positive samples to develop computational methods capable of identifying miRNA-target interactions. miRTarBase is now available on http://miRTarBase.mbc.nctu.edu.tw/, and is updated frequently by continuously surveying research articles.
URI: http://dx.doi.org/10.1093/nar/gkq1107
http://hdl.handle.net/11536/26050
ISSN: 0305-1048
DOI: 10.1093/nar/gkq1107
期刊: NUCLEIC ACIDS RESEARCH
Volume: 39
Issue: 
起始頁: D163
結束頁: D169
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