標題: Functional Cells Cultured on Microcarriers for Use in Regenerative Medicine Research
作者: Sun, Li-Yi
Lin, Shinn-Zong
Li, Yuan-Sheng
Harn, Horng-Jyh
Chiou, Tzyy-Wen
生物科技學系
Department of Biological Science and Technology
關鍵字: Microcarriers;Stem cell;Expansion;Transplantation
公開日期: 1-Jan-2011
摘要: Microcarriers have been successfully used for many years for growing anchorage-dependent cells and as a means of delivering cells for tissue repair. When cultured on microcarriers, the number of anchorage-dependent cells, including primary cells, can easily be scaled up and controlled to generate the quantities of cells necessary for therapeutic applications. Recently, stem cell technology has been recognized as a powerful tool in regenerative medicine, but adequate numbers of stem cells that retain their differentiation potential are still difficult to obtain. For anchorage-dependent stem cells, however, microcarrier-based suspension culture using various types of microcarriers has proven to be a good alternative for effective ex vivo expansion. In this article, we review studies reporting the expansion, differentiation, or transplantation of functional anchorage-dependent cells that were expanded with the microcarrier culture system. Thus, the implementation of technological advances in biodegradable microcarriers, the bead-to-bead transfer process, and appropriate stem cell media may soon foster the ability to produce the numbers of stem cells necessary for cell-based therapies and/or tissue engineering.
URI: http://dx.doi.org/10.3727/096368910X532792
http://hdl.handle.net/11536/26023
ISSN: 0963-6897
DOI: 10.3727/096368910X532792
期刊: CELL TRANSPLANTATION
Volume: 20
Issue: 1
起始頁: 49
結束頁: 62
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