標題: Doxorubicin-Loaded Nanosized Micelles of a Star-Shaped Poly(epsilon-Caprolactone)-Polyphosphoester Block Co-polymer for Treatment of Human Breast Cancer
作者: Nguyen-Van Cuong
Hsieh, Ming-Fa
Chen, Yung-Tsung
Liau, Ian
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Star-shaped co-polymer;multidrug resistance;micelles;doxorubicin;poly(epsilon-caprolactone);poly(ethyl ethylene phosphate)
公開日期: 2011
摘要: Star-shaped co-polymers based on the backbone of poly(epsilon-caprolactone) were synthesized by a ring-opening reaction using pentaerythritol as initiator and Sn(Oct)(2) as catalyst. The star-shaped poly(epsilon-caprolactone) polymer was then chain extended with a terminal block of poly(ethyl ethylene phosphate) to form a copolymer, poly(epsilon-caprolactone)-poly(ethyl ethylene phosphate), when using the cyclic ethyl ethylene phosphate monomer. The amphiphilic block co-polymers can self-assemble into nanoscopic micelles with a mean diameter of 150 nm and a spherical shape. Additionally, the prepared micelles did not induce hemolysis and nitric oxide production in vitro based on nitric oxide, hemolytic tests and MTT assays. The hydrophobic micellar cores encapsulated doxorubicin (DOX) in an aqueous solution with a loading efficiency of 55.2%. The in vitro release of DOX from DOX-loaded micelles was pH dependent. DOX-loaded micelles present significantly enhanced cytotoxicity to both MCF-7/drug-sensitive and MCF-7/drug-resistant cells after second incubation. Moreover, results of confocal microscopy and flow cytometry of DOX-loaded micelles demonstrate the feasibility of this delivery system for effective therapy of drug-resistant tumours. (C) Koninklijke Brill NV, Leiden, 2011
URI: http://hdl.handle.net/11536/25968
http://dx.doi.org/10.1163/092050610X510533
ISSN: 0920-5063
DOI: 10.1163/092050610X510533
期刊: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume: 22
Issue: 11
起始頁: 1409
結束頁: 1426
顯示於類別:期刊論文


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  1. 000291407000001.pdf