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dc.contributor.authorMaezawa, Yasuyoen_US
dc.contributor.authorOkano, Kazunorien_US
dc.contributor.authorMatsubara, Mieen_US
dc.contributor.authorMasuhara, Hiroshien_US
dc.contributor.authorHosokawa, Yoichirohen_US
dc.date.accessioned2014-12-08T15:37:36Z-
dc.date.available2014-12-08T15:37:36Z-
dc.date.issued2011-02-01en_US
dc.identifier.issn1387-2176en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s10544-010-9476-4en_US
dc.identifier.urihttp://hdl.handle.net/11536/25849-
dc.description.abstractWhen nerve growth factor (NGF) is interacted with PC12 cells derived from rat pheochromocytoma, they are partially differentiated into neuron-like cells with neurites. In this work, PC12 cells differentiated by NGF were selectively isolated using a localized impulsive force in a mu m-scale area, which was generated by focusing an infrared femtosecond laser into a cell culture medium. In order to evaluate the ability of the isolation method, differentiated and undifferentiated cells were isolated and their morphological changes after the isolation were compared. In both cases, their neurites were once contracted and some of them gradually regenerated day by day. When differentiated cells were isolated, the percentage of differentiated cells with regenerated neurites, 6 h after the isolation, was about 3.3 times higher than that when undifferentiated ones were isolated. This result was compared with a control trypsin experiment. In the comparison, it was indicated that the same degree of cell function was maintained when the present isolation method was used.en_US
dc.language.isoen_USen_US
dc.subjectFemtosecond laseren_US
dc.subjectLaser manufacturingen_US
dc.subjectImpulsive forceen_US
dc.subjectCell Isolationen_US
dc.subjectCell differentiationen_US
dc.titleMorphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive forceen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/s10544-010-9476-4en_US
dc.identifier.journalBIOMEDICAL MICRODEVICESen_US
dc.citation.volume13en_US
dc.citation.issue1en_US
dc.citation.spage117en_US
dc.citation.epage122en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000286627000012-
dc.citation.woscount5-
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