標題: Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae
作者: Wang, Yu-Kuo
Huang, Sheng-Cih
Wu, Yi-Fang
Chen, Yu-Ching
Chen, Wen-Hung
Lin, Yan-Ling
Nayak, Manoswini
Lin, Yan Ren
Li, Thomas Tien-Hsiung
Wu, Tung-Kung
生物科技學系
Department of Biological Science and Technology
公開日期: 1-二月-2011
摘要: Vibrio hollisae, a halophilic species recently reclassified as Grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. One important pathogenic Vibrio factor, thermostable direct haemolysin (TDH), has been purified and crystallized in two crystal forms using the vapour-diffusion method. The crystals belonged to an orthorhombic space group, with unit-cell parameters a = 104.8, b = 112.4, c = 61.3 angstrom and a = 122.9, b = 123.3, c = 89.8 angstrom. The crystals contained either four or eight molecules per asymmetric unit, with predicted solvent contents of 49.4 and 46.3% and Matthews coefficients (V(M)) of 2.4 and 2.3 angstrom(3) Da(-1), respectively. These crystals were suitable for structure determination, which would yield structural details related to the cytotoxicity and oligomeric structure of this pore-forming toxin.
URI: http://dx.doi.org/10.1107/S1744309110050219
http://hdl.handle.net/11536/25791
ISSN: 1744-3091
DOI: 10.1107/S1744309110050219
期刊: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
Volume: 67
Issue: 
起始頁: 224
結束頁: 227
顯示於類別:期刊論文


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