標題: CaNdt80 is involved in drug resistance in Candida albicans by regulating CDR1
作者: Chen, CG
Yang, YL
Shih, HI
Su, CL
Lo, HJ
生物科技學系
Department of Biological Science and Technology
公開日期: 1-Dec-2004
摘要: Overexpression of CDR1, an efflux pump, is one of the major mechanisms contributing to drug resistance in Candida albicans. CDR1 p-lacZ was constructed and transformed into a Saccharomyces cerevisiae strain so that the lacZ gene could be used as the reporter to monitor the activity of the CDR1 promoter. Overexpression of CaNDT80, the C albicans homolog of S. cerevisiae NDT80, increases the beta-galactosidase activity of the CDR1 p-lacZ construct in S. cerevisiae. Furthermore, mutations in CaNDT80 abolish the induction of CDR1 expression by antifungal agents in C albicans. Consistently, the Candt80/Candt80 mutant is also more susceptible to antifungal drugs than the wild-type strain. Thus, the gene for CaNdt80 may be the first gene among the regulatory factors involved in drug resistance in C. albicans whose function has been identified.
URI: http://dx.doi.org/10.1128/AAC.48.12.4505-4512.2004
http://hdl.handle.net/11536/25574
ISSN: 0066-4804
DOI: 10.1128/AAC.48.12.4505-4512.2004
期刊: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume: 48
Issue: 12
起始頁: 4505
結束頁: 4512
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