標題: Characterization of intact Penicillium spores by matrix-assisted laser desorption/ionization mass spectrometry
作者: Chen, HY
Chen, YC
應用化學系
Department of Applied Chemistry
公開日期: 2005
摘要: The fungal spores of Penicillium expansum, P. chrysogenum, P. citrinum, P. digitatum, P. italicum, and P. pinophilum were characterized by using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOFMS). These fungal spores are frequently found in grain and fruit. The mass spectra of these six species were directly obtained from the intact spores without any pretreatment. The results obtained indicate that 2,5-dihydroxybenzoicacid and sinapinic acid are suitable matrices for the analysis of Penicillium spores. Characteristic ions representing the different species were obtained with sufficiently high reproducibility that these ions can be employed to identify the different fungal species. On the basis of these characteristic ions obtained from these authentic Penicillium spores, the approach was applied to characterize the fungal species contaminating the surfaces of fruit. It was demonstrated that the fungal spores directly scratched from the surfaces of fruit contaminated by unknown fungi can be rapidly identified using MALDI-TOFMS analysis without any tedious pretreatment. Copyright (c) 2005 John Wiley & Sons, Ltd.
URI: http://hdl.handle.net/11536/25424
http://dx.doi.org/10.1002/rcm.2229
ISSN: 0951-4198
DOI: 10.1002/rcm.2229
期刊: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume: 19
Issue: 23
起始頁: 3564
結束頁: 3568
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