標題: Multiphoton microscopy in defining liver function
作者: Thorling, Camilla A.
Crawford, Darrell
Burczynski, Frank J.
Liu, Xin
Liau, Ian
Roberts, Michael S.
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: multiphoton microscopy;second harmonic generation;fluorescence lifetime imaging microscopy;fibrosis;hepatocellular carcinoma;mitochondria;drug transport;nanoparticles
公開日期: 1-Sep-2014
摘要: Multiphoton microscopy is the preferred method when in vivo deep-tissue imaging is required. This review presents the application of multiphoton microscopy in defining liver function. In particular, multiphoton microscopy is useful in imaging intracellular events, such as mitochondrial depolarization and cellular metabolism in terms of NAD(P) H changes with fluorescence lifetime imaging microscopy. The morphology of hepatocytes can be visualized without exogenously administered fluorescent dyes by utilizing their autofluorescence and second harmonic generation signal of collagen, which is useful in diagnosing liver disease. More specific imaging, such as studying drug transport in normal and diseased livers are achievable, but require exogenously administered fluorescent dyes. If these techniques can be translated into clinical use to assess liver function, it would greatly improve early diagnosis of organ viability, fibrosis, and cancer. (C) The Authors.
URI: http://dx.doi.org/10.1117/1.JBO.19.9.090901
http://hdl.handle.net/11536/25373
ISSN: 1083-3668
DOI: 10.1117/1.JBO.19.9.090901
期刊: JOURNAL OF BIOMEDICAL OPTICS
Volume: 19
Issue: 9
起始頁: 0
結束頁: 0
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