標題: Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus
作者: Hsieh, Yin-Cheng
Chiu, Hsi-Ho
Huang, Yen-Chieh
Fun, Hoong-Kun
Lu, Chia-Yu
Li, Yaw-Kuen
Chen, Chun-Jung
交大名義發表
應用化學系
National Chiao Tung University
Department of Applied Chemistry
公開日期: 1-Sep-2014
摘要: Glycosyltransferases (GTs), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. Glycosyltransferase-1 from Bacillus cereus (BcGT-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (UDP-glucose) as the sugar donor. BcGT-1 (molecular mass 45.5 kDa) has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction of BcGT-1 crystals to 2.10 angstrom resolution, the crystal belonged to space group P1, with unit-cell parameters a = 54.56, b = 84.81, c = 100.12 A, alpha = 78.36, beta = 84.66, gamma = 84.84 degrees. Preliminary analysis indicates the presence of four BcGT-1 molecules in the asymmetric unit with a solvent content of 50.27%.
URI: http://dx.doi.org/10.1107/S2053230X14014629
http://hdl.handle.net/11536/25200
ISSN: 1744-3091
DOI: 10.1107/S2053230X14014629
期刊: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume: 70
Issue: 
起始頁: 1228
結束頁: 1231
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