標題: Expression of Survivin and p53 Modulates Honokiol-Induced Apoptosis in Colorectal Cancer Cells
作者: Lai, Ying-Jiun
Lin, Chien-I
Wang, Chia-Lin
Chao, Jui-I
交大名義發表
生物科技學系
分子醫學與生物工程研究所
National Chiao Tung University
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: HONOKIOL;SURVIVIN;p53;APOPTOSIS;COLORECTAL CANCER
公開日期: 1-Nov-2014
摘要: Honokiol is a small biphenolic compound, which exerts antitumor activities; however, the precise mechanism of honokiol-induced apoptosis in the human colorectal cancer cells remains unclear. Here, we show that survivin and p53 display the opposite role on the regulation of honokiol-induced apoptosis in the human colorectal cancer cells. Honokiol induced the cell death and apoptosis in various colorectal cancer cell lines. Moreover, honokiol elicited the extrinsic death receptor pathway of DR5 and caspase 8 and the intrinsic pathway of caspase 9. The common intrinsic and extrinsic downstream targets of activated caspase 3 and PARP protein cleavage were induced by honokiol. Interestingly, honokiol reduced anti-apoptotic survivin protein and gene expression. Transfection with a green fluorescent protein (GFP)-survivin-expressed vector increased the colorectal cancer cell viability and resisted the honokiol-induced apoptosis. Meantime, honokiol increased total p53 and the phosphorylated p53 proteins at Ser15 and Ser46. The p53-wild type colorectal cancer cells were exhibited greater cytotoxicity, apoptosis and survivin reduction than the p53-null cancer cells after treatment with honokiol. Together, these findings demonstrate that the existence of survivin and p53 can modulate the honokiol-induced apoptosis in the human colorectal cancer cells. J. Cell. Biochem. 115: 1888-1899, 2014. (c) 2014 Wiley Periodicals, Inc.
URI: http://dx.doi.org/10.1002/jcb.24858
http://hdl.handle.net/11536/25149
ISSN: 0730-2312
DOI: 10.1002/jcb.24858
期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
Volume: 115
Issue: 11
起始頁: 1888
結束頁: 1899
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