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dc.contributor.authorHosokawa, Masahitoen_US
dc.contributor.authorAndo, Masahiroen_US
dc.contributor.authorMukai, Shoichiroen_US
dc.contributor.authorOsada, Kyokoen_US
dc.contributor.authorYoshino, Tomokoen_US
dc.contributor.authorHamaguchi, Hiro-oen_US
dc.contributor.authorTanaka, Tsuyoshien_US
dc.date.accessioned2014-12-08T15:36:42Z-
dc.date.available2014-12-08T15:36:42Z-
dc.date.issued2014-08-19en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac501591den_US
dc.identifier.urihttp://hdl.handle.net/11536/25058-
dc.description.abstractA straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for the rapid, direct, and quantitative determination of lipid molecules from living cells using single-cell Raman imaging. In vivo localization of lipids in the form of triacylglycerol (TAG) within oleaginous microalga and their molecular compositions are monitored with high spatial resolution in a non-destructive and label-free manner. This method can provide quantitative and real-time information on compositions, chain lengths, and degree of unsaturation of fatty acids in living cells for improving the cultivating parameters or for determining the harvest timing during large-scale cultivations for microalgal lipid accumulation toward biodiesel production. Therefore, this technique is a potential tool for in vivo lipidomics for understanding the dynamics of lipid metabolisms in various organisms.en_US
dc.language.isoen_USen_US
dc.titleIn Vivo Live Cell Imaging for the Quantitative Monitoring of Lipids by Using Raman Microspectroscopyen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac501591den_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume86en_US
dc.citation.issue16en_US
dc.citation.spage8224en_US
dc.citation.epage8230en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000340701500029-
dc.citation.woscount0-
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