標題: High-Resolution Mapping of Chromatin Packaging in Mouse Embryonic Stem Cells and Sperm
作者: Carone, Benjamin R.
Hung, Jui-Hung
Hainer, Sarah J.
Chou, Min-Te
Carone, Dawn M.
Weng, Zhiping
Fazzio, Thomas G.
Rando, Oliver J.
生物資訊及系統生物研究所
Institude of Bioinformatics and Systems Biology
公開日期: 1-Jul-2014
摘要: Mammalian embryonic stem cells (ESCs) and sperm exhibit unusual chromatin packaging that plays important roles in cellular function. Here, we extend a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-binding proteins genome-wide in murine ESCs and sperm. In ESCs, we recover well-characterized features of chromatin such as promoter nucleosome depletion and further identify widespread footprints of sequence-specific DNA-binding proteins such as CTCF, which we validate in knockdown studies. We document global differences in nuclease accessibility between ESCs and sperm, finding that the majority of histone retention in sperm preferentially occurs in large gene-poor genomic regions, with only a small subset of nucleosomes being retained over promoters of developmental regulators. Finally, we describe evidence that CTCF remains associated with the genome in mature sperm, where it could play a role in organizing the sperm genome.
URI: http://dx.doi.org/10.1016/j.devcel.2014.05.024
http://hdl.handle.net/11536/24904
ISSN: 1534-5807
DOI: 10.1016/j.devcel.2014.05.024
期刊: DEVELOPMENTAL CELL
Volume: 30
Issue: 1
起始頁: 11
結束頁: 22
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