標題: Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum
作者: Chen, Mei-Hsiu
Hu, Chao-Kai
Chen, Pei-Ru
Chen, Yu-Shan
Sun, Jui-Sheng
Chen, Ming-Hong
生物科技學系
Department of Biological Science and Technology
關鍵字: Estradiol;Ligamentum flavum;Matrix metalloproteinases;Collagen;Elastin
公開日期: 15-Jul-2014
摘要: Background: Estradiol plays an important role in the regulation of collagen metabolism. Deficiency of estradiol has been reported to be associated with the degeneration of many connective tissues. However, the association of estradiol and hypertrophy of the ligamentum flavum was seldom explored. Therefore, we studied the effects of estradiol on cultured cells from the ligamentum flavum. Methods: Primary cultures of human ligamentum flavum cells obtained from surgical specimens of 14 patients undergoing spinal surgery were used to investigate the effect of estradiol on cell proliferation and the expression of collagen, elastin, and matrix metalloproteinases. Downstream pathways of estrogen receptor underlying the regulation of metalloproteinases were also investigated. Results: In our study, we revealed the existence of estrogen receptors on both female and male ligamentum flavum cells with a gender difference. 17 beta-estradiol increased early (24 hours) proliferation of ligamentum flavum cells in a dose dependent manner and the effect could not be seen when the cell density increased. Estradiol with a concentration of 10-9 M decreased collagen levels and increased the expression of MMP-13. Adding an antagonist of PI3K downstream pathway could reverse the expression of MMP-13 caused by estradiol. Conclusions: The results implied estradiol regulated the expression of MMP-13 via PI3K pathway and contributed to the homeostasis of extracellular matrix in the ligamentum flavum.
URI: http://dx.doi.org/10.1186/1471-2474-15-238
http://hdl.handle.net/11536/24874
ISSN: 1471-2474
DOI: 10.1186/1471-2474-15-238
期刊: BMC MUSCULOSKELETAL DISORDERS
Volume: 15
Issue: 
結束頁: 
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