標題: A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites
作者: Vankayala, Raviraj
Chiang, Chi-Shiun
Chao, Jui-I
Yuan, Chiun-Jye
Lin, Shyr-Yeu
Hwang, Kuo Chu
生物科技學系
Department of Biological Science and Technology
關鍵字: Gene transfection;Gene therapy;Nanocarriers;Zebrafish;Cytotoxicity;Carbon nanoparticles
公開日期: 1-Sep-2014
摘要: Gene therapy provides a new hope for previously "incurable" diseases. Low gene transfection efficiency, however, is the bottle-neck to the success of gene therapy. It is very challenging to develop non-viral nanocarriers to achieve ultra-high gene transfection efficiencies. Herein, we report a novel design of "tight binding-but-detachable" lipid-nanoparticle composite to achieve ultrahigh gene transfection efficiencies of 60 similar to 82%, approaching the best value (similar to 90%) obtained using viral vectors. We show that Fe@CNPs nanoparticles coated with LP-2000 lipid molecules can be used as gene carriers to achieve ultra-high (60-80%) gene transfection efficiencies in HeLa, U-87MG, and TRAMP-C1 cells. In contrast, Fe@CNPs having surface-covalently bound N,N,N-trimethyl-N-2-methacryloxyethyl ammonium chloride (TMAEA) oligomers can only achieve low (23-28%) gene transfection efficiencies. Similarly ultrahigh gene transfection/expression was also observed in zebrafish model using lipid-coated Fe@CNPs as gene carriers. Evidences for tight binding and detachability of DNA from lipid-nanoparticle nanocarriers will be presented. (C) 2014 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.biomaterials.2014.06.016
http://hdl.handle.net/11536/24815
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2014.06.016
期刊: BIOMATERIALS
Volume: 35
Issue: 28
起始頁: 8261
結束頁: 8272
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