標題: In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy
作者: Miyaoka, Rimi
Hosokawa, Masahito
Ando, Masahiro
Mori, Tetsushi
Hamaguchi, Hiro-o
Takeyama, Haruko
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Raman microspectroscopy;in situ detection;antibiotics;secondary metabolites;actinomycetes
公開日期: 1-五月-2014
摘要: The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes-Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling.
URI: http://dx.doi.org/10.3390/md12052827
http://hdl.handle.net/11536/24734
ISSN: 1660-3397
DOI: 10.3390/md12052827
期刊: MARINE DRUGS
Volume: 12
Issue: 5
起始頁: 2827
結束頁: 2839
顯示於類別:期刊論文


文件中的檔案:

  1. 000337160500027.pdf