標題: Spacer-enhanced chymotrypsin-activated peptide-functionalized gold nanoparticle probes: a rapid assay for the diagnosis of pancreatitis
作者: Yeh, Fang-Yuan
Tseng, I-Hua
Chuang, Shu-Hung
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
公開日期: 2014
摘要: Pancreatitis is the inflammation of the pancreas. Chymotrypsin, an indicator of pancreatic function, could serve as a biomarker for the diagnosis of pancreatitis. A gold nanoparticle (AuNP)-based fluorescence assay was fabricated in this study to assay the activity of chymotrypsin. Peptides labeled with fluorophores were conjugated onto AuNPs as a chymotrypsin-activated peptide-functionalized AuNP probe (AuNPs-peptide probe). The detection sensitivity of the AuNPs-peptide probe toward proteolytic activity was significantly increased by using spacer-enhanced peptides with specifically designed lengths and charges. The limit of detection of the designed AuNPs-peptide probe decreased, enabling detection at the pM level within a 15 min detection time. The AuNPs-peptide probe was used to evaluate chymotrypsin activity as an indicator of acute pancreatitis (AP) in a mouse model induced by cerulein challenge. The fecal chymotrypsin activity in cerulein-induced AP mice was significantly lower than that observed in controls. This is the first study to use an AuNPs-peptide probe for the diagnosis of pancreatitis using fecal specimens.
URI: http://hdl.handle.net/11536/24585
http://dx.doi.org/10.1039/c4ra00258j
ISSN: 2046-2069
DOI: 10.1039/c4ra00258j
期刊: RSC ADVANCES
Volume: 4
Issue: 42
起始頁: 22266
結束頁: 22276
顯示於類別:期刊論文


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