標題: A PDMS-Based Cylindrical Hybrid Lens for Enhanced Fluorescence Detection in Microfluidic Systems
作者: Lin, Bor-Shyh
Yang, Yu-Ching
Ho, Chong-Yi
Yang, Han-Yu
Wang, Hsiang-Yu
影像與生醫光電研究所
Institute of Imaging and Biomedical Photonics
關鍵字: microfluidic systems;optical components;hybrid lens;fluorescence measurement;polydimethylsiloxane (PDMS)
公開日期: 1-二月-2014
摘要: Microfluidic systems based on fluorescence detection have been developed and applied for many biological and chemical applications. Because of the tiny amount of sample in the system; the induced fluorescence can be weak. Therefore, most microfluidic systems deploy multiple optical components or sophisticated equipment to enhance the efficiency of fluorescence detection. However, these strategies encounter common issues of complex manufacturing processes and high costs. In this study; a miniature, cylindrical and hybrid lens made of polydimethylsiloxane (PDMS) to improve the fluorescence detection in microfluidic systems is proposed. The hybrid lens integrates a laser focusing lens and a fluorescence collecting lens to achieve dual functions and simplify optical setup. Moreover, PDMS has advantages of low-cost and straightforward fabrication compared with conventional optical components. The performance of the proposed lens is first examined with two fluorescent dyes and the results show that the lens provides satisfactory enhancement for fluorescence detection of Rhodamine 6G and Nile Red. The overall increments in collected fluorescence signal and detection sensitivity are more than 220% of those without lens, and the detection limits of Rhodamine 6G and Nile red are lowered to 0.01 g/mL and 0.05 g/mL, respectively. The hybrid lens is further applied to the detection of Nile red-labeled Chlorella vulgaris cells and it increases both signal intensity and detection sensitivity by more than 520%. The proposed hybrid lens also dramatically reduces the variation in detected signal caused by the deviation in incident angle of excitation light.
URI: http://dx.doi.org/10.3390/s140202967
http://hdl.handle.net/11536/24508
ISSN: 1424-8220
DOI: 10.3390/s140202967
期刊: SENSORS
Volume: 14
Issue: 2
起始頁: 2967
結束頁: 2980
顯示於類別:期刊論文


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