標題: Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds
作者: Hsu, Tzu-Chia
Liu, Kuang-Kai
Chang, Huan-Cheng
Hwang, Eric
Chao, Jui-I
生物科技學系
生物資訊及系統生物研究所
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
公開日期: 16-五月-2014
摘要: Nanodiamond is a promising carbon nanomaterial developed for biomedical applications. Here, we show fluorescent nanodiamond (FND) with the biocompatible properties that can be used for the labeling and tracking of neuronal differentiation and neuron cells derived from embryonal carcinoma stem (ECS) cells. The fluorescence intensities of FNDs were increased by treatment with FNDs in both the mouse P19 and human NT2/D1 ECS cells. FNDs were taken into ECS cells; however, FNDs did not alter the cellular morphology and growth ability. Moreover, FNDs did not change the protein expression of stem cell marker SSEA-1 of ECS cells. The neuronal differentiation of ECS cells could be induced by retinoic acid (RA). Interestingly, FNDs did not affect on the morphological alteration, cytotoxicity and apoptosis during the neuronal differentiation. Besides, FNDs did not alter the cell viability and the expression of neuron-specific marker beta-III-tubulin in these differentiated neuron cells. The existence of FNDs in the neuron cells can be identified by confocal microscopy and flow cytometry. Together, FND is a biocompatible and readily detectable nanomaterial for the labeling and tracking of neuronal differentiation process and neuron cells from stem cells.
URI: http://dx.doi.org/10.1038/srep05004
http://hdl.handle.net/11536/24422
ISSN: 2045-2322
DOI: 10.1038/srep05004
期刊: SCIENTIFIC REPORTS
Volume: 4
Issue: 
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