標題: Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions
作者: Chien, Wei-Ting
Liang, Chien-Fu
Yu, Ching-Ching
Lin, Chien-Hung
Li, Si-Peng
Primadona, Indah
Chen, Yu-Ju
Mong, Kwok Kong T.
Lin, Chun-Cheng
應用化學系
Department of Applied Chemistry
公開日期: 2014
摘要: A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine. By incorporating glycosyltransferases, oligo-LacNAcs and their sialylated analogs were synthesized.
URI: http://hdl.handle.net/11536/24312
http://dx.doi.org/10.1039/c4cc01227e
ISSN: 1359-7345
DOI: 10.1039/c4cc01227e
期刊: CHEMICAL COMMUNICATIONS
Volume: 50
Issue: 43
起始頁: 5786
結束頁: 5789
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