標題: Ion Intensity and Thermal Proton Transfer in Ultraviolet Matrix-Assisted Laser Desorption/Ionization
作者: Lu, I-Chung
Lee, Chuping
Chen, Hui-Yuan
Lin, Hou-Yu
Hung, Sheng-Wei
Dyakov, Yuri A.
Hsu, Kuo-Tung
Liao, Chih-Yu
Lee, Yin-Yu
Tseng, Chien-Ming
Lee, Yuan-Tseh
Ni, Chi-Kung
應用化學系
Department of Applied Chemistry
公開日期: 17-四月-2014
摘要: The ionization mechanism of ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI) was investigated by measuring the total cation intensity (not including sodiated and potasiated ions) as a function of analyte concentration (arginine, histidine, and glycine) in a matrix of 2,4,6-trihydroxyacetophenone (THAP). The total ion intensity increased up to SS times near the laser fluence threshold as the arginine concentration increased from 0% to 1%. The increases were small for histidine, and a minimal increase occurred for glycine. Time-resolved fluorescence intensity was employed to investigate how analytes affected the energy pooling of the matrix. No detectable energy pooling was observed for pure THAP and THAP/analyte mixtures. The results can be described by using a thermal proton transfer model, which suggested that thermally induced proton transfer is crucial in the primary ion generation in UV-MALDI.
URI: http://dx.doi.org/10.1021/jp5008076
http://hdl.handle.net/11536/24231
ISSN: 1520-6106
DOI: 10.1021/jp5008076
期刊: JOURNAL OF PHYSICAL CHEMISTRY B
Volume: 118
Issue: 15
起始頁: 4132
結束頁: 4139
顯示於類別:期刊論文


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