標題: Sensitivity evaluation of NBD-SCN towards cysteine/homocysteine and its bioimaging applications
作者: Chen, Yen-Hao
Tsai, Jia-Chun
Cheng, Tsan-Hwang
Yuan, Shyng-Shiou
Wang, Yun-Ming
生物科技學系
Department of Biological Science and Technology
關鍵字: Cysteine;Homocysteine;ICT;NBD;Fluorescent probe
公開日期: 15-六月-2014
摘要: A push-pull fluorogenic reagent, NBD-SCN, was applied for specific detection of cysteine (Cys) and homocysteine (Hcy). Replacing thiocyanato group with Cys/Hcy increased the push-pull characteristic of the probe and resulted in emission of fluorescence. The fluorescent response of the probe toward Cys/Hcy was significantly higher than toward glutathione and other amino acids. The probe showed a 470-and 745-fold fluorescence enhancement at 550 nm and detection limit of 2.99 and 1.43 nM for Cys and Hcy, respectively. Time-dependent fluorescence assays showed that the fluorescence intensity reached a plateau within 20 s after addition of Cys and within 10 min after addition of Hcy. Furthermore, the fluorescence images of Cys/Hcy in Raw 264.7 cells were obtained after adding this probe to the cells. These results indicate that NBD-SCN not only possesses good selectivity and sensitivity for Cys/Hcy but also can penetrate cells for Cys/Hcy bioimaging. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2014.01.009
http://hdl.handle.net/11536/24179
ISSN: 0956-5663
DOI: 10.1016/j.bios.2014.01.009
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 56
Issue: 
起始頁: 117
結束頁: 123
顯示於類別:期刊論文


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