標題: Thermal stability and folding kinetics analysis of disordered protein, securin
作者: Chu, Hsueh-Liang
Chen, Tzu-Hsuan
Wu, Chang-You
Yang, Yao-Chen
Tseng, Shin-Hua
Cheng, Tsai-Mu
Ho, Li-Ping
Tsai, Li-Yun
Li, Hsing-yuan
Chang, Chia-Seng
Chang, Chia-Ching
生物科技學系
Department of Biological Science and Technology
關鍵字: Securin;Disordered protein;Thermal stability;Folding kinetics
公開日期: 1-Mar-2014
摘要: Lacking a stable tertiary structure, intrinsically disordered proteins (IDPs) possess particular functions in cell regulation, signaling, and controlling pathways. The study of their unique structural features, thermal stabilities, and folding kinetics is intriguing. In this study, an identified IDP, securin, was used as a model protein. By using a quasi-static five-step (on-path) folding process, the function of securin was restored and analyzed by isothermal titration calorimetry. Fluorescence spectroscopy and particle size analysis indicated that securin possessed a compact hydrophobic core and particle size. The glass transition of securin was characterized using differential scanning microcalorimetry. Furthermore, the folding/unfolding rates (k(obs)) of securin were undetectable, implying that the folding/unfolding rate is very fast and that the conformation of securin is sensitive to solvent environmental change. Therefore, securin may fold properly under specific physiological conditions. In summary, the thermal glass transition behavior and undetectable k(obs) of folding/unfolding reactions may be two of the indices of IDP.
URI: http://dx.doi.org/10.1007/s10973-013-3598-x
http://hdl.handle.net/11536/24018
ISSN: 1388-6150
DOI: 10.1007/s10973-013-3598-x
期刊: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
Volume: 115
Issue: 3
起始頁: 2171
結束頁: 2178
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