標題: A facile ratiometric fluorescent chemodosimeter for hydrazine based on Ing-Manske hydrazinolysis and its applications in living cells
作者: Raju, Mandapati V. Ramakrishnam
Prakash, Epperla Chandra
Chang, Huan-Cheng
Lin, Hong-Cheu
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: Charge transfer;Fluorescent probe;Ing-Manske hydrazinolysis;pH effects;Semi-empirical calculations;Time resolved photoluminescence spectra
公開日期: 1-Apr-2014
摘要: A facile and sensitive fluorescent probe for hydrazine was successfully constructed, displaying excellent colorimetric and ratiometric responses towards hydrazine via Ing-Manske hydrazinolysis conditions in semi-aqueous buffer solution. Semi-empirical calculations as well as spectroscopic results revealed the signalling mechanism of the current probe under hydrazinolysis conditions, in which hydrazine exclusively deprotected the phthalimide group by an intermediate of phthalhydrazide. Extensive screening of pH effects on the probe with the aid of proton nuclear magnetic resonance and mass spectrometry supported the distinctive and diverse ratiometric responses under hydrazinolysis and basic hydrolysis conditions. Time resolved photoluminescence measurements of the probe further confirmed its discernible ratiometric responses probed at respective wavelengths. A distinctive ratiometric response under basic hydrolysis conditions and a successful utilization of probe towards hydrazine detection in living cells are demonstrated. (C) 2013 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.dyepig.2013.11.015
http://hdl.handle.net/11536/23747
ISSN: 0143-7208
DOI: 10.1016/j.dyepig.2013.11.015
期刊: DYES AND PIGMENTS
Volume: 103
Issue: 
起始頁: 9
結束頁: 20
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