標題: Real time monitoring of accelerated chemical reactions by ultrasonication-assisted spray ionization mass spectrometry
作者: Lin, Shu-Hsuan
Lo, Ta-Ju
Kuo, Fang-Yin
Chen, Yu-Chie
應用化學系
Department of Applied Chemistry
關鍵字: ultrasonication;real time monitoring;mass spectrometry;electrospray ionization;steroids
公開日期: 1-一月-2014
摘要: Ultrasonication has been used to accelerate chemical reactions. It would be ideal if ultrasonication-assisted chemical reactions could be monitored by suitable detection tools such as mass spectrometry in real time. It would be helpful to clarify reaction intermediates/products and to have a better understanding of reaction mechanism. In this work, we developed a system for ultrasonication-assisted spray ionization mass spectrometry (UASI-MS) with an similar to 1.7 MHz ultrasonic transducer to monitor chemical reactions in real time. We demonstrated that simply depositing a sample solution on the MHz-based ultrasonic transducer, which was placed in front of the orifice of a mass spectrometer, the analyte signals can be readily detected by the mass spectrometer. Singly and multiply charged ions from small and large molecules, respectively, can be observed in the UASI mass spectra. Furthermore, the ultrasonic transducer used in the UASI setup accelerates the chemical reactions while being monitored via UASI-MS. The feasibility of using this approach for real-time acceleration/monitoring of chemical reactions was demonstrated. The reactions of Girard T reagent and hydroxylamine with steroids were used as the model reactions. Upon the deposition of reactant solutions on the ultrasonic transducer, the intermediate/product ions are readily generated and instantaneously monitored using MS within 1 s. Additionally, we also showed the possibility of using this reactive UASI-MS approach to assist the confirmation of trace steroids from complex urine samples by monitoring the generation of the product ions. Copyright (c) 2014 John Wiley & Sons, Ltd.
URI: http://dx.doi.org/10.1002/jms.3319
http://hdl.handle.net/11536/23628
ISSN: 1076-5174
DOI: 10.1002/jms.3319
期刊: JOURNAL OF MASS SPECTROMETRY
Volume: 49
Issue: 1
起始頁: 50
結束頁: 56
顯示於類別:期刊論文


文件中的檔案:

  1. 000329877800008.pdf