標題: Fluorescence Intrinsic Characterization of Excitation-Emission Matrix Using Multi-Dimensional Ensemble Empirical Mode Decomposition
作者: Chang, Chi-Ying
Chang, Chia-Chi
Hsiao, Tzu-Chien
分子醫學與生物工程研究所
資訊工程學系
Institute of Molecular Medicine and Bioengineering
Department of Computer Science
關鍵字: excitation-emission matrix (EEM);multi-dimensional ensemble empirical mode decomposition (MEEMD)
公開日期: 1-十一月-2013
摘要: Excitation-emission matrix (EEM) fluorescence spectroscopy is a noninvasive method for tissue diagnosis and has become important in clinical use. However, the intrinsic characterization of EEM fluorescence remains unclear. Photobleaching and the complexity of the chemical compounds make it difficult to distinguish individual compounds due to overlapping features. Conventional studies use principal component analysis (PCA) for EEM fluorescence analysis, and the relationship between the EEM features extracted by PCA and diseases has been examined. The spectral features of different tissue constituents are not fully separable or clearly defined. Recently, a non-stationary method called multi-dimensional ensemble empirical mode decomposition (MEEMD) was introduced; this method can extract the intrinsic oscillations on multiple spatial scales without loss of information. The aim of this study was to propose a fluorescence spectroscopy system for EEM measurements and to describe a method for extracting the intrinsic characteristics of EEM by MEEMD. The results indicate that, although PCA provides the principal factor for the spectral features associated with chemical compounds, MEEMD can provide additional intrinsic features with more reliable mapping of the chemical compounds. MEEMD has the potential to extract intrinsic fluorescence features and improve the detection of biochemical changes.
URI: http://dx.doi.org/10.3390/ijms141122436
http://hdl.handle.net/11536/23465
ISSN: 1422-0067
DOI: 10.3390/ijms141122436
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 14
Issue: 11
起始頁: 22436
結束頁: 22448
顯示於類別:期刊論文


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  1. 000328624400074.pdf