標題: A comparison of two 16S rRNA gene-based PCR primer sets in unraveling anammox bacteria from different environmental samples
作者: Han, Ping
Huang, Yu-Tzu
Lin, Jih-Gaw
Gu, Ji-Dong
環境工程研究所
Institute of Environmental Engineering
關鍵字: Anammox;Detection;Diversity;PCR primer;Abundance;Distribution
公開日期: 1-Dec-2013
摘要: Two 16S rRNA gene-based PCR primer sets (Brod541F/Amx820R and A438f/A684r) for detecting anammox bacteria were compared using sediments from Mai Po wetlands (MP), the South China Sea (SCS), a freshwater reservoir (R2), and sludge granules from a wastewater treatment plant (A2). By comparing their ability in profiling anammox bacteria, the recovered diversity, community structure, and abundance of anammox bacteria among all these diverse samples indicated that A438f/A684r performed better than Brod541F/Amx820R in retrieving anammox bacteria from these different environmental samples. Five Scalindua subclusters (zhenghei-I, SCS-I, SCS-III, arabica, and brodae) dominated in SCS whereas two Scalindua subclusters (zhenghei-II and wagneri) and one cluster of Kuenenia dominated in MP. R2 showed a higher diversity of anammox bacteria with two new retrieved clusters (R2-New-1 and R2-New-2), which deserves further detailed study. The dominance of Brocadia in sample A2 was supported by both of the primer sets used. Results collectively indicate strongly niche-specific community structures of anammox bacteria in different environments, and A438f/A684r is highly recommended for screening anammox bacteria from various environments when dealing with a collection of samples with diverse physiochemical characteristics.
URI: http://dx.doi.org/10.1007/s00253-013-5305-z
http://hdl.handle.net/11536/23228
ISSN: 0175-7598
DOI: 10.1007/s00253-013-5305-z
期刊: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume: 97
Issue: 24
起始頁: 10521
結束頁: 10529
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