標題: Spatially reinforced Au nano-cavities as a reaction nano-reservoir for trace analysis of DNA hybridization
作者: Yao, Chih-Kai
Liao, Jiunn-Der
Lin, Chih-Heng
Yang, Yuh-Shyong
Yu, Sheng-Hong
Yang, Jung-Wei
生物科技學系
Department of Biological Science and Technology
關鍵字: Spatially reinforced Au nano-cavities;Nano-reservoir;DNA hybridization;Raman shift;Labeling-free
公開日期: 1-二月-2014
摘要: Spatially reinforced Au nano-cavities (SR-nAu) with reduced tip-to-tip displacement create strongly localized surface plasmon resonance by Raman scattering. Within SR-nAu, the nano-cavity structure with high aspect ratio was optimized as the nano-reservoir for the distinction of DNA hybridization responses from specific DNA sequences of Avian influenza virus. In the nano-reservoir, the suspended or immobilized probe, its complementary and noncomplementary target sequences were characterized at very low concentration through enhanced Raman spectra. The presence of Raman shift at 738 cm(-1), the characteristic breathing mode of thymine, and the change of relative peaks intensity at 1603 and 1554 cm(-1) before and after hybridization were particularly taken as the major indications to validate the match or mismatch state of DNA sequences. Through a labeling-free means, and from the evidence provided by specific complementary and noncomplementary DNA sequences, the recognition capability of nano-reservoir can be down to subnano-molar concentration (10(-10) M). (C) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.snb.2013.09.120
http://hdl.handle.net/11536/23188
ISSN: 0925-4005
DOI: 10.1016/j.snb.2013.09.120
期刊: SENSORS AND ACTUATORS B-CHEMICAL
Volume: 191
Issue: 
起始頁: 219
結束頁: 226
顯示於類別:期刊論文


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