標題: Highly electrostatically-induced detection selectivity and sensitivity for a colloidal biosensor made of chitosan nanoparticle decorated with a few bare-surfaced gold nanorods
作者: Jean, Ren-Der
Cheng, Wei-Da
Hsiao, Meng-Hsuan
Chou, Fu-Hsuan
Bow, Jong-Shing
Liu, Dean-Mo
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: Biosensor;Electrostatic interaction;Gold nanorod;Selectivity;Human serum albumin;Lysozyme
公開日期: 15-二月-2014
摘要: Metallic nanoparticles have been utilized as an analytical tool to detecting a wide variety of organic analytes. Among them, gold nanoparticles demonstrating outstanding surface plasmonic resonance property have been well recognized and received wide attention for plasmon-based sensing applications. However, in literature, gold-based nanosensor has to be integrated with specific "ligand" molecule in order to gain molecular recognition ability. However, "ligand" molecules, included proteins, peptides, nucleic acids, etc. are expensive and vulnerable to environmental change, in the meantime, anchoring procedure of the "ligand" molecules to gold surface may be cost-ineffective and endangered to the ligand's activity, making a final analytic probe less reliable and risk in production capability. Here, we develop a new approach by designing a colloid-type sensor using a few "bare" Au nanorods deposited on the surface of a colloidal chitosan carrier. By tuning the solution pH, the resulting colloidal nanoprobe is capable of detecting proteins, i.e., human serum albumin and lysozyme, with high specificity and sensitivity. This new approach allows a new type of the molecular probes to be well manipulated to monitor important biomolecules for medical detection, diagnosis, and bioengineering applications. (C) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2013.08.047
http://hdl.handle.net/11536/23187
ISSN: 0956-5663
DOI: 10.1016/j.bios.2013.08.047
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 52
Issue: 
起始頁: 111
結束頁: 117
顯示於類別:期刊論文


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