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dc.contributor.authorYeh, Yuan-Mingen_US
dc.contributor.authorHuang, Kuo-Yangen_US
dc.contributor.authorGan, Ruei-Chi Richieen_US
dc.contributor.authorHuang, Hsien-Daen_US
dc.contributor.authorWang, Tzu-Chien V.en_US
dc.contributor.authorTang, Petrusen_US
dc.date.accessioned2014-12-08T15:33:02Z-
dc.date.available2014-12-08T15:33:02Z-
dc.date.issued2013-10-01en_US
dc.identifier.issn1684-1182en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.jmii.2012.07.010en_US
dc.identifier.urihttp://hdl.handle.net/11536/22996-
dc.description.abstractBackground/Purpose(s): Trichomoniasis caused by Trichomonas vaginalis is the most common non-viral sexually transmitted infection. Morphological transformation from the trophozoite stage to the amoeboid or pseudocyst stage is crucial for T. vaginalis infection and survival. Protein phosphorylation is a key post-translational modification involved in the regulation of several biological processes in various prokaryotes and eukaryotes. More than 880 protein kinases have been identified in the T. vaginalis genome. However, little is known about the phosphorylation of specific proteins and the distribution of phosphorylated proteins in different stages of the morphological transformation of T. vaginalis. Methods: To obtain a more comprehensive understanding of the T. vaginalis phosphoproteome, we analyzed phosphorylated proteins in the three morphological stages using titanium dioxide combined with LC-MS/MS. Results: A total of 93 phosphopeptides originating from 82 unique proteins were identified. Among these proteins, 21 were detected in all stages, 29 were identified in two different stages, and 32 were stage specific. Conclusion: Identification of stage-specific phosphorylated proteins indicates that phosphorylation of these proteins may play a key role in the morphological transformation of T. vaginalis. Copyright (C) 2012, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectPhosphoproteomeen_US
dc.subjectTitanium dioxide enrichmenten_US
dc.subjectTrichomonas vaginalisen_US
dc.titlePhosphoproteome profiling of the sexually transmitted pathogen Trichomonas vaginalisen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jmii.2012.07.010en_US
dc.identifier.journalJOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTIONen_US
dc.citation.volume46en_US
dc.citation.issue5en_US
dc.citation.spage366en_US
dc.citation.epage373en_US
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.identifier.wosnumberWOS:000326213500008-
dc.citation.woscount1-
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