標題: Phosphoproteome profiling of the sexually transmitted pathogen Trichomonas vaginalis
作者: Yeh, Yuan-Ming
Huang, Kuo-Yang
Gan, Ruei-Chi Richie
Huang, Hsien-Da
Wang, Tzu-Chien V.
Tang, Petrus
生物資訊及系統生物研究所
Institude of Bioinformatics and Systems Biology
關鍵字: Phosphoproteome;Titanium dioxide enrichment;Trichomonas vaginalis
公開日期: 1-Oct-2013
摘要: Background/Purpose(s): Trichomoniasis caused by Trichomonas vaginalis is the most common non-viral sexually transmitted infection. Morphological transformation from the trophozoite stage to the amoeboid or pseudocyst stage is crucial for T. vaginalis infection and survival. Protein phosphorylation is a key post-translational modification involved in the regulation of several biological processes in various prokaryotes and eukaryotes. More than 880 protein kinases have been identified in the T. vaginalis genome. However, little is known about the phosphorylation of specific proteins and the distribution of phosphorylated proteins in different stages of the morphological transformation of T. vaginalis. Methods: To obtain a more comprehensive understanding of the T. vaginalis phosphoproteome, we analyzed phosphorylated proteins in the three morphological stages using titanium dioxide combined with LC-MS/MS. Results: A total of 93 phosphopeptides originating from 82 unique proteins were identified. Among these proteins, 21 were detected in all stages, 29 were identified in two different stages, and 32 were stage specific. Conclusion: Identification of stage-specific phosphorylated proteins indicates that phosphorylation of these proteins may play a key role in the morphological transformation of T. vaginalis. Copyright (C) 2012, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.
URI: http://dx.doi.org/10.1016/j.jmii.2012.07.010
http://hdl.handle.net/11536/22996
ISSN: 1684-1182
DOI: 10.1016/j.jmii.2012.07.010
期刊: JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
Volume: 46
Issue: 5
起始頁: 366
結束頁: 373
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