標題: Continuous production of high-purity fructooligosaccharides and ethanol by immobilized Aspergillus japonicus and Pichia heimii
作者: Sheu, Dey Chyi
Chang, Jan Yi
Wang, Chung Yih
Wu, Chang Ta
Huang, Chen Ji
生物科技學系
Department of Biological Science and Technology
關鍵字: Aspergillus japonicus;Cell immobilization;Ethanol;Fructooligosaccharides;Pichia heimii
公開日期: 1-十一月-2013
摘要: High-purity fructooligosaccharides (FOS) were produced from sucrose by an innovative process incorporating immobilized Aspergillus japonicus and Pichia heimii cells. Intracellular FTase of A. japonicus converted sucrose into FOS and glucose, and P. heimii fermented glucose mainly into ethanol. The continuous production of FOS was carried out using a tanks-in-series bioreactor consisting of three stirred tanks. When a solution composed of 1 g L-1 yeast extract and 300 g L-1 sucrose was fed continuously to the bioreactor at a dilution rate of 0.1 h(-1), FOS at a purity of up to 98.2 % could be achieved and the value-added byproduct ethanol at 79.6 g L-1 was also obtained. One gram of sucrose yielded 0.62 g FOS and 0.27 g ethanol. This immobilized dual-cell system was effective for continuous production of high-purity FOS and ethanol for as long as 10 days.
URI: http://dx.doi.org/10.1007/s00449-013-0949-8
http://hdl.handle.net/11536/22959
ISSN: 1615-7591
DOI: 10.1007/s00449-013-0949-8
期刊: BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume: 36
Issue: 11
起始頁: 1745
結束頁: 1751
顯示於類別:期刊論文


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