標題: Analysis of single algal cells by combining mass spectrometry with Raman and fluorescence mapping
作者: Fagerer, Stephan R.
Schmid, Thomas
Ibanez, Alfredo J.
Pabst, Martin
Steinhoff, Robert
Jefimovs, Konstantins
Urban, Pawel L.
Zenobi, Renato
交大名義發表
National Chiao Tung University
公開日期: 2013
摘要: In order to investigate metabolic properties of single cells of freshwater algae (Haematococcus pluvialis), we implement matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in combination with microspectroscopic mapping. Straightforward coupling of these two detection platforms was possible thanks to the self-aliquoting properties of micro-arrays for mass spectrometry (MAMS). Following Raman and fluorescence imaging, the isolated cells were covered with a MALDI matrix for targeted metabolic analysis by MALDI-MS. The three consecutive measurements carried out on the same cells yielded complementary information. Using this method, we were able to study the encystment of H. pluvialis - by monitoring the adenosine triphosphate (ATP) to adenosine diphosphate (ADP) ratio during the build-up of astaxanthin in the cells as well as the release of beta-carotene, the precursor of astaxanthin, into the cytosol.
URI: http://hdl.handle.net/11536/22904
http://dx.doi.org/10.1039/c3an01135f
ISSN: 0003-2654
DOI: 10.1039/c3an01135f
期刊: ANALYST
Volume: 138
Issue: 22
起始頁: 6732
結束頁: 6736
顯示於類別:期刊論文


文件中的檔案:

  1. 000325819300004.pdf