標題: COLLAGEN VI PROTECTS AGAINST NEURONAL APOPTOSIS ELICITED BY ULTRAVIOLET IRRADIATION VIA AN AKT/PHOSPHATIDYLINOSITOL 3-KINASE SIGNALING PATHWAY
作者: Cheng, I. H.
Lin, Y. -C.
Hwang, E.
Huang, H. -T.
Chang, W. -H.
Liu, Y. -L.
Chao, C. -Y.
生物科技學系
生物資訊及系統生物研究所
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
關鍵字: collagen VI;neuroprotection;UV;Akt/PI3K;NeurotoxQ
公開日期: 2-六月-2011
摘要: Collagen VI, one of the extracellular matrix proteins, has been implicated in regulating cell proliferation and reducing apoptosis in several different systems. However, the role of collagen VI in the central nervous system remains unclear. In this manuscript, we demonstrated that upon ultraviolet (UV) irradiation, mouse primary hippocampal neurons specifically up-regulate the expression of Col6a1, Col6a2, and Col6a3 mRNA and secreted collagen VI protein. Augmentation of collagen VI mRNA and protein after UV irradiation may have a neuroprotective role as suggested by the fact that extracellular supplying soluble collagen VI protein, but not other collagen proteins, reduced UV induced DNA damage, mitochondria dysfunction, and neurite shrinkage. We also tried to determine the signaling molecules that mediate the protective effect of collagen VI via Western blot and inhibitor analysis. After collagen VI treatment, UV irradiated neurons increased phosphorylation of Akt and decreased phosphorylation of JNK. Inhibiting Akt/Phosphatidylinositol 3-kinases (PI3K) pathway diminished the protective effect of collagen VI. Our study suggested a potential protective mechanism by which neurons up-regulate collagen VI production under stress conditions to activate Akt/PI3K anti-apoptotic signaling pathway. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.neuroscience.2011.03.057
http://hdl.handle.net/11536/22792
ISSN: 0306-4522
DOI: 10.1016/j.neuroscience.2011.03.057
期刊: NEUROSCIENCE
Volume: 183
Issue: 
起始頁: 178
結束頁: 188
顯示於類別:期刊論文


文件中的檔案:

  1. 000290748300017.pdf