Title: The effect of coenzyme Q10 included by gamma-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy
Authors: Nishida, Tatsuro
Kaino, Tomohiro
Ikarashi, Ryo
Nakata, Daisuke
Terao, Keiji
Ando, Masahiro
Hamaguchi, Hiro-o
Kawamukai, Makoto
Yamamoto, Tatsuyuki
Department of Applied Chemistry
Institute of Molecular science
Keywords: Fission yeast;Growth;Metabolic activity;Coenzyme Q10;Cyclodextrin;Raman spectroscopy
Issue Date: 24-Sep-2013
Abstract: The inclusion complex of coenzyme Q10 (CoQ10) by gamma-cyclodextrin (gamma-CD), CoQ10-CD complex, was recently developed. The addition of the CoQ10-CD complex recovered the growth of a fission yeast mutant strain, Delta dps1, which otherwise cannot grow well due to the lack of coenzyme Q producing ability. However, the oxygen consumption rate of this strain was not restored by the addition of the CoQ10-CD complex. The addition of two other anti-oxidative reagents, glutathione and ascorbic acid, also recovered the growth of the Delta dps1 strain as well. These results indicated that the recovery of the growth of Delta dps1 was brought about by the anti-oxidative property of CoQ10. The intensity of Raman spectra of Delta dps1 at 1602 cm(-1), which is prominently observed for the wild type of the fission yeast, was compared between before and after addition of the CoQ10-CD complex. The signal was very weakly observed for Delta dps1 and did not increase in intensity by the addition of the CoQ10-CD complex. These results suggested the recovery of the growth of Delta dps1 was brought about not by the restoration of respiration function of Delta dps1 but by the anti-oxidative property of CoQ10 to result in the decrease in the oxidative stress. (c) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.molstruc.2013.05.066
ISSN: 0022-2860
DOI: 10.1016/j.molstruc.2013.05.066
Volume: 1048
Begin Page: 375
End Page: 381
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