標題: The effect of coenzyme Q10 included by gamma-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy
作者: Nishida, Tatsuro
Kaino, Tomohiro
Ikarashi, Ryo
Nakata, Daisuke
Terao, Keiji
Ando, Masahiro
Hamaguchi, Hiro-o
Kawamukai, Makoto
Yamamoto, Tatsuyuki
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Fission yeast;Growth;Metabolic activity;Coenzyme Q10;Cyclodextrin;Raman spectroscopy
公開日期: 24-九月-2013
摘要: The inclusion complex of coenzyme Q10 (CoQ10) by gamma-cyclodextrin (gamma-CD), CoQ10-CD complex, was recently developed. The addition of the CoQ10-CD complex recovered the growth of a fission yeast mutant strain, Delta dps1, which otherwise cannot grow well due to the lack of coenzyme Q producing ability. However, the oxygen consumption rate of this strain was not restored by the addition of the CoQ10-CD complex. The addition of two other anti-oxidative reagents, glutathione and ascorbic acid, also recovered the growth of the Delta dps1 strain as well. These results indicated that the recovery of the growth of Delta dps1 was brought about by the anti-oxidative property of CoQ10. The intensity of Raman spectra of Delta dps1 at 1602 cm(-1), which is prominently observed for the wild type of the fission yeast, was compared between before and after addition of the CoQ10-CD complex. The signal was very weakly observed for Delta dps1 and did not increase in intensity by the addition of the CoQ10-CD complex. These results suggested the recovery of the growth of Delta dps1 was brought about not by the restoration of respiration function of Delta dps1 but by the anti-oxidative property of CoQ10 to result in the decrease in the oxidative stress. (c) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.molstruc.2013.05.066
http://hdl.handle.net/11536/22514
ISSN: 0022-2860
DOI: 10.1016/j.molstruc.2013.05.066
期刊: JOURNAL OF MOLECULAR STRUCTURE
Volume: 1048
Issue: 
起始頁: 375
結束頁: 381
顯示於類別:期刊論文


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  1. 000323468500051.pdf